National Parks are the gems of the Last Great Places. 49 National Parks are found in the lower 48 states, and collectively capture examples of over half of all ecosystems. 45 of these Parks are ranked in the 2500 Last Great Places; 10 are found in the top 50.
| National Parks in top 50 Last Great Places | Ecoregion |
| Glacier National Park | Canadian Rocky Mountains |
| Olympic National Park | Pacific Northwest Coast |
| Grand Canyon National Park | Colorado Plateau |
| Death Valley National Park | Mojave Desert |
| Big Bend National Park | Chihuahuan Desert |
| Yellowstone National Park | Utah-Wyoming Mtns |
| Everglades National Park | Tropical Florida |
| Isle Royale National Park | Great Lakes |
| Mount Rainier National Park | West Cascades |
| Great Basin National Park | Great Basin |
